MS is one of the most common diseases of the CNS, the incidence rate is 30 cases per 100,000 people globally but in Europe it reaches 80 cases per 100,000. Multiple sclerosis (MS) is a chronic inflammatory disease, where demyelination specifically affects the central nervous system (CNS) 1. ![]() By exploring the CSF lipidome we demonstrate usefulness of broad-range shotgun lipidomic analysis as a fast and reliable method of biomarker discovery in patients with demyelinating neurological disorders that might be a valuable diagnostic complement to existing examinations. We found strong positive correlation between total protein content and lipid concentrations in both diseases. As a common feature of demyelination, we identified upregulated levels of lipid metabolic intermediates. In contrast, in MS, the accumulation of the myelin-derived cerebrosides represented a specific feature of demyelination. For the GBS group, the fourfold elevation in the total lipid content was a discriminatory and a newly identified feature of PNS demyelination. The quantification of 222 CSF lipid molecular species revealed characteristic changes in the absolute and relative lipid concentrations in MS and GBS compared to the controls. In the present study high sensitivity shotgun mass spectrometry was used to characterise the CSF lipidome of patients with MS, GBS and controls with non-demyelinating diseases. ![]() Cerebrospinal fluid (CSF) is one of the most valuable sources of diagnostic biomarkers in neurological diseases. Multiple sclerosis (MS) and Guillain –Barré syndrome (GBS) are demyelinating disorders affecting the central nervous system and peripheral nervous system (PNS), respectively.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |